Sr. Scientist I - Cell & Molecular Biologist

Job Description
Key Responsibilities
Design, execute, &
interpret critical experiments to answer scientific questions.
Generate &
test hypothesis by designing, performing, &
interpreting laboratory-based experiments.
Read literature critically &
analyze genomic data tosupport these activities.
Develop in vitro &
in vivo screening assays in collaboration with therapeutic area scientists by generating cell lines, developing lentiviral &
RNP delivery methods, &
integrating &
optimizing screening protocols.
Responsible for transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, &
performing CRISPR screens in neuronal &
glial cell types.
Perform pooled &
arrayed CRISPR screens with FACS-based molecular &
functional assays on immortalized cell lines, primary cells, &
iPSC-derived cells.
Work closely with NGS &
bioinformatics scientists to process genomic samples, analyze screening results, &
generate screening hitsfor follow-up studies.
Investigate novel screening hits in the context of disease biology by generating &
testing hypothesis using a variety of biochemical, molecular, cellular, &
genomic assays.
Utilize molecular analytic methods including DNA/RNA isolation, PCR/qPCR, molecular cloning, genotyping, &
/or western blot.
Map candidate genes to known disease genetic networks through experimental &
bioinformatic approaches.
Learn, understand, &
master new experimentaltechniques, &
act as a resource or mentor for others. Perform RNA-sequencing, RNAi (shRNA/siRNA), &
CRISPR gene editing.
Collaborate and communicate with cross-functional scientists on experimental designs and results and scientific goals.
Report research progress to management on a regular basis, provide project updates to cross-functional project teams, and provide scientific presentations to department and other internal scientific forums.
Seek and advance opportunities to present research results at external scientific conferences &
publish high-impact articles in peer-reviewed journals.